Thermal, chemical and chemothermal denaturation of yeast enolase
نویسندگان
چکیده
منابع مشابه
Protective role of trehalose in thermal denaturation of yeast pyrophosphatase.
Trehalose, a disaccharide of glucose, is accumulated in yeast cytosol when this organism is submitted to a stress condition. Recently it was shown that the level of trehalose increase up to 15 times when yeast cells are submitted to heat shock (De Virgilio et al., 1991). In this report we give evidence how trehalose may play an important role on the stress-survival of yeasts when submitted to a...
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The DNA molecule can take various conformational forms. Investigations focus mainly on the so-called ‘B-form’, schematically drawn in the famous paper by Watson and Crick [1]. This is the usual form of DNA in a biological environment and is the only form that is stable in an aqueous environment. Other forms, however, can teach us much about DNA. They have the same nucleotide base pairs for ‘bui...
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The conformational stabilities of eight proteins in terms of the free energy differences between the native "folded" state of the protein and its "unfolded" state were determined at 298 K by two methods: chemical denaturation at 298 K and extrapolation to 298 K of the thermal denaturation results at high temperature. The proteins were expressed in Escherichia coli from the Haemophilus influenza...
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Green fluorescent protein (GFP) is an unusually stable autofluorescent protein that is increasingly being exploited for many applications. In this report, we have used fluorescence spectroscopy to study the effect of pH on the denaturation of GFP with sodium dodecyl sulfate (SDS), urea, and heat. Surprisingly, SDS (up to 0.5%) did not have any significant effect on the fluorescence of GFP at pH...
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ژورنال
عنوان ژورنال: Spectroscopy
سال: 2003
ISSN: 0712-4813,1875-922X
DOI: 10.1155/2003/941801